Methylation profiling of human gDNA using Oxford Nanopore Adaptive Sampling

This project explores the use of Oxford Nanopore adaptive sampling for DNA methylation profiling of age-associated and body fluid-specific markers. By selectively enriching CpG sites in real-time, this approach enables comprehensive methylation analysis without the need for specialized biochemical treatments. Our findings demonstrate the feasibility of adaptive sampling for accurate methylation detection, with strong consistency to whole-genome bisulfite sequencing. This study lays the groundwork for nanopore-based methodologies in forensic applications. For more details, refer to our published study: https://doi.org/10.1016/j.fsigen.2024.103048.