Text S1 - 2-Butanol and Butanone Production in <i>Saccharomyces cerevisiae</i> through Combination of a B<sub>12</sub> Dependent Dehydratase and a Secondary Alcohol Dehydrogenase Using a TEV-Based Expression System
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Sequences of the constructs. Sequences of the PCR-fragment used to integrate construct of pduGH at a locus 1000 bp downstream of the DAK2 stop-codon and plasmid constructs for SADH and pduCDE expression. The integrative construct and SADH plasmid have a TDH3 promoter and pduCDE plasmid has a TPI1 promoter. All the constructs have a 3′UTR/terminator region from ADH1. (DOCX)
创建时间：
2014-07-23
