Epigenome analysis of DNA samples from children's peripheral blood leukocyte

Genome-wide DNA methylation profiling of white blood cells was conducted using the Illumina Infinium Human Methylation 850K Genechip. According to the internal exposure level of deoxynivalenol, 32 participants were divided into two groups (high exposure DON group and low exposure DON group). Samples included 16 peripheral blood samples from children with high DON exposure and 16 peripheral blood samples from children with low DON exposure. About 3 ml of peripheral blood was taken for DNA extraction. White blood cells (WBCs) were isolated from blood specimens. Then total DNA was extracted from WBCs. Bisulphite converted DNA from the 32 samples were hybridised to the Illumina Infinium Human Methylation 850K Genechip