Expression data from mouse peritoneal macrophages

Biologic functions involved in innate immune response of macrophages rely on the precise regulation of kinds of immune molecular. In the LPS treatment procession, the macrophages are activated following a tightly controlled genetic programme where specific sets of genes are up-regulated or down-regulated. We used microarrays to detail the global programme of gene expression underlying LPS treatment and identified distinct classes of up-regulated and down-regulated genes during this process. Mouse peritoneal macrophages were selected with/without LPS treatment for 6 hours for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain expression profiles. We selected macrophages according to LPS treatment at two time-points: uninfected macrophage(Mock) and LPS treatment for 6 hour macrophages(LPS).