Epigenetic dysregulation of energy homeostasis drives aortic valve stenosis that is treatable with metformin [Cut & Tag]

This study aimed to investigate epigenetic regulation in murine aortic valves by profiling H3K27ac enrichment in control and Hdac3-deficient valves, as well as HDAC3 and phosphorylated HDAC3 (Ser424) occupancy in control valves. Using CUT&Tag, we assessed genome-wide changes in enhancer activity and HDAC3 binding to identify regulatory elements and pathways disrupted by Hdac3 deletion in valvular interstitial cells. These data provide insight into the role of HDAC3 and its phosphorylation in maintaining epigenetic homeostasis and gene expression programs critical for aortic valve integrity. CUT&Tag on Aortic valves from 12-week-old control and Hdac3-knockout mice