Urotensin II Activates Sensory-cutaneous Pathway to Promote Itch and Cutaneous Inflammation

In order to study the effect of UII on the transcription level regulation of itch-related genes in sensory neurons and keratinocytes, and to apply UII in mouse dorsal root ganglion neuronal cells and keratinocytes, the study showed that UII significantly upregulated the expression of various characteristics of atopic dermatitis and itch-related genes, such as Fasl, Mrgprx1, Amy2a3, INHBA, IL36G, STEAP2, etc. Overall design: To investigate the effect of UII activation on gene expression regulation in mouse dorsal root ganglion neuronal cells and human primary keratinocytes. RNA sequencing of 6 mouse dorsal root ganglion neuronal cell samples and 6 human keratinocytes samples. They were 3 UII-treated mouse dorsal root ganglion neuronal cell samples, 3 from untreated control group cells, in addition to 3 UII-treated human primary keratinocytes for 24 h and 3 cells from untreated control cells.