The V5-V6 variable region of the 16S rRNA gene was used to amplify the bacteria communities Raw sequence reads

While previous studies have examined the effects of land use on streams and soil microbiota, few have compared both soil and water compartments within the same riparian systems. In this study, we characterized bacterial composition in six small to moderate-sized streams in both urban and agricultural land use areas using 16S rRNA gene amplicon sequencing and related this to measured physicochemical variables in these environments.