Renal carcinoma/kidney progenitor cell chimera organoid as a novel tumorigenesis gene discovery model

Three-dimensional (3D) organoids provide a new way to model various diseases, including cancer. We made use of recently developed kidney-organ-primordia tissue-engineering technologies to create novel renal organoids for cancer gene discovery. We then tested whether our novel assays can be used to examine kidney cancer development. First, we identified the transcriptomic profiles of quiescent embryonic mouse metanephric mesenchyme (MM) and of MM in which the nephrogenesis program had been induced ex vivo. The transcriptome profiles were then compared to the profiles of tumor biopsies from renal cell carcinoma (RCC) patients, and control samples from the same kidneys. Certain signature genes were identified that correlated in the developmentally induced MM and RCC, including components of the caveolar-mediated endocytosis signaling pathway. An efficient siRNA-mediated knockdown (KD) of Bnip3, Gsn, Lgals3, Pax8, Cav1, Egfr or Itgb2 gene expression was achieved in mouse RCC (Renca) cells. The live-cell imaging analysis revealed inhibition of cell migration and cell viability in the gene-KD Renca cells in comparison to Renca controls. Upon siRNA treatment, the transwell invasion capacity of Renca cells was also inhibited. Finally, we mixed the nephron progenitors with yellow fluorescent protein (YFP)-expressing Renca cells to establish chimera organoids. Strikingly, we found that the Bnip3-, Cav1- and Gsn-KD Renca-YFP+ cells as a chimera with the MM in 3D organoid rescued, in part, the RCC-mediated inhibition of the nephrogenesis program during epithelial tubules formation. Altogether, our research indicates that comparing renal ontogenesis control genes to the genes involved in kidney cancer may provide new growth-associated gene screens and that 3D RCC-MM chimera organoids can serve as a novel model with which to investigate the behavioral roles of cancer cells within the context of emergent complex tissue structures. Embryonic kidneys were dissected from CD1 embryos at E11.5 and the ureteric bud was removed from the nephron-progenitor/stem-cell-containing MM (Saxén, 1987). The MM cells were washed twice with cell culture medium, placed on Nuclepore polycarbonate membrane , and a piece of embryonic dorsal spinal cord (E11.5) was glued onto the other filer side as a robust tubulogenesis inducer. The conjugate was cultured in 37°C, at 5% CO2, for 96 h, snap frozen in liquid nitrogen, and stored at −80°C until used for RNA purification. Collectively, 40 freshly prepared control and induced MM were processed for the oligonucleotide gene-chip analysis. The analyses were conducted in triplicates.