FLAG-TdIF1_ChIP-seq_293T

TdIF1 was originally identified as a protein that directly binds to terminal deoxynucleotidyl transferase, TdT. Recently, through SELEX analysis, we showed that TdIF1 recognizes a specific DNA sequence motif, 5???-TGCATG-3??? and up-regulates the expression of RAB20. Other target genes of TdIF1, however, remain to be identified. Here, we conducted ChIP-seq, and determined TdIF1-binding sequences (TdIF1-invivoBM) on the human chromosomes. Gene ontology analysis and RT-qPCR method revealed the enrichment of some candidate targets in the genes involved in regulation of ossification or cellular signaling pathways. To determine TdIF1-regulated genes, ChIP-seq was conducted with FLAG-TdIF1 overexpressing 293T cells using anti-DYKDDDDK antibody. ChIPed DNA was sequenced by SOLiD4.