Analysis of genomes, transcriptomes and intestinal microorganisms of pond smelt (KAP210041)

1. Genome sequencing of smelt (Hypomesus nipponensis). Smelt genome sequencing. Smelt genome assembly. Improvement of genome assembly using IsoSeq. Mitochondrial DNA analysis and comparison between species. Confirmation of draft genome. Gene prediction and annotation. Development of smelt relative species comparison marker 2. Transcriptome analysis (2 tissue, more than 10 samples). RNA-Seq comparison by tissue (Liver and muscle). Comparison of each tissue's gene expression by sex. Comparison of each tissue's gene expression by temperature (high/low) 3. Smelt microbiome analysis (More than 10 samples). Smelt skin/gut microbiome analysis (based on 16S rRNA). Analysis of effects of fish intake (using mice, more than 2 group)