RNA-seq and ATAC-seq data of scaffold 3D bioprinted GBM

MSC can acquired characteristics of Cancer associated fibroblasts (CAF) by being cultured with conditioned medium from GBM cultures and thus are called MSCCAF. We co-cultured MSCCAF with patient derived GBM in a scaffold 3D bioprinted model. We studied the response to current GBM therapy (e.g. Temozolomide +/- radiation) on the co-cultures by bulk transcriptomic (RNA-sequencing) and epigenetic (ATAC-seq) analyses. RNA-seq Cells were harvested, cryopreserved and sent to Active Motif (https://www.activemotif.com) where RNA-seq were performed: RNA extraction, polyA+ RNA enrichment, directional library generation, QC of NGS library and next-generation sequencing using the Illumina platform. ATAC-seq Cells were harvested, cryopreserved and sent to Active Motif (https://www.activemotif.com/) where ATAC-seq were performed following the protocols from Buenrostro et al. 2013 Nat. Methods and Corces et al. 2017 Nat. Methods.