Efficient remediation of heavy metal contaminated soil by combination of ryegrass with a multifunctional bacterial consortium

We collected soil from polymetallic mine wastelands and non-contaminated soil in the campus to conduct pot experiments. By planting ryegrass (Lolium perenne) and applying a mixed bacterial consortium composed of phosphate-solubilizing bacteria, nitrogen-fixing bacteria and sulfate-reducing bacteria, the remediation efficiency of this method was evaluated, and the internal mechanism was explored by amplicon sequencing and gene prediction.