RNA-sequencing and metabolomics data of "A clinical-stage direct Nrf2 activator suppresses osteoclast differentiation via iron-ornithine axis"

This dataset includes the raw RNA-sequencing (counts) and metabolomics data generated in the study. All the RNA-sequencing and metabolomic assays were performed on bone marrow derived macrophages of wildtype or Nrf2-KO C57BL6J mice. For RNA sequencing, BMDMs were seeded on 6-well plates with 1×10^6 cells per well. Each group had five replicates from different mice and cells were collected after three days of culture and intervention. Total RNA was extracted with TRIzol (Takara, Japan). For the metabolomic assay, BMDMs were cultured in 10-cm dishes with 1×10^7 cells per dish. After intervention, cells were collected in prechilled 80% methanol and were subjected to three freeze-thaw cycles in liquid nitrogen. LC-MS/MS was used for the untargeted metabolomic assay. All assays were performed by Novogene (Beijing, China).