RNA-seq of cytosolic and chromatin-associated transcripts following TNFα and Spt5 KD. Homo sapiens

We examined the effects of TNFα and Spt5, the major DSIF subunit, on nascent and mature transcripts using RNA-Seq of chromatin-associated and cytoplasmic transcripts. Overall design: RNA was extracted from the cytosolic and chromatin fractions of control and Spt5 KD cells that were treated with TNFα for 1 hour