Nick Ferenz, Patricia Wadsworth (2012) CIL:40368, Sus scrofa domestica, kidney epithelial cell. CIL. Dataset

Time lapse series of a porcine kidney LLCPK1 cell stably expressing photoactivable GFP-tubulin. Fluorescence was activated at prophase in a vertical strip perpendicular to the spindle orientation. Microtubules in the activated strip show a variety of motions both toward and away from spindle poles. This contrasts with the exclusively poleward motion of spindle microtubules seen at metaphase (and illustrated in the grouped movie CIL:40266). A movie prepared from the time series is included in this group of images.
See Fig 1A in: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002

本数据集呈现了一组稳定表达可光激活GFP-微管蛋白的猪肾LLCPK1细胞的延时摄影系列图像。荧光激活发生在垂直于纺锤体方向的子午线区域。激活区域内微管的表现出向纺锤体极和远离纺锤体极的多种运动，与中期观察到的纺锤体微管仅朝向纺锤体极的运动形成鲜明对比（详见分组电影CIL:40266中的图示）。本数据集包含由该时间序列制作的电影。参见NP Ferenz和P Wadsworth于2007年发表的论文《哺乳细胞中前期微管阵列呈现类似流变的行为》。Mol Biol Cell 18:3993-4002，图1A。