A novel single TCR gamma chain fused with antibody-based binding domain facilitates T cell cytotoxicity for potent anti-tumor response without forming TCR-CD3 complex

To test the hypothesis that the activation and regulatory signaling in γ-TCRγ-T cells could be dissimilar from the conventional TCR-T, we stimulated anti-PD-L1 δ-TCRγδ- and γ-TCRγ-T cells engineered from three different donors, and then compared the genome-wide transcriptome profiles by RNA sequencing (RNA-Seq). Comparative gene expression profiling analysis of RNA-seq data for γ-TCRγ-T cells (gamma9 group) and δ-TCRγδ-T cells (g9d2 group) after antigen stimulation