Change of 5hmC and 5mC landscapes in A2058-TET2WT cells cultured in high-g and normal-g

We report that high glucose can have a detrimental effect on the stability of TET2 protein, and thereby reduce the genome-wide 5hmC content. We use MeDIP and hMeDIP followed by sequencing to identify the change of 5hmC and 5mC landscapes in A2058-TET2WT over expressing cells cultured in high and normal glucose concentrations. Overall design: A2058-TET2WT over expressing cells were cultured in high-g and normal-g for days. Their 5mC and 5hmC were profiled by MeDIP and hMeDIP sequencing.