The role of RUNX2 and BHLHE40 in pathologically relevant CD4-positive tissue-resident T-cells in Crohn's disease. (CUT&RUN)

Tissue-resident T-cells (TRM) are deeply involved in immune memory at the site of inflammation. Here, we identified two key transcription factors, RUNX2 and BHLHE40 as regulators of pathologically relevant CD4-positive TRM in the inflamed gut mucosa of Crohn’s disease patients. Chromatin profiles in human PBMC-derived CD4 positive T cells overexpressing RUNX2 or BHLHE40 though Lentiviral infection was performed by CUT&RUN Assay using anti-RUNX2 antibody and BHLHE40 antibody, respectively.