Effects of oxysterol Oxy186 and Oxy210 on A549 xenograft tumor growth

To examine the efficacy of oxysterols in inhibiting tumor growth in vivo, we tested Oxy186 and Oxy210 in subcutaneous xenograft tumor models constructed with A549 cells in nude mice. After the tumor size reached an average of ~100 mm3, the compounds were administered daily via oral gavage at 50 mg/kg. We found that Oxy186 started to inhibit tumor growth at day 8, and significantly reduced the tumor volume from day 11 to day18 of treatment, compared to that of vehicle control. Surprisingly, Oxy210 did not show such inhibitory effect. Total RNA from control and Oxy186-treated or Oxy210-treated xenografts were extracted, libraries were prepared with NEBNext UltraTM II RNA Librart Prep Kit from RNAs with RIN>8.0, and RNAseq were performed using Illumina Novaseq 6000 (Novogene).