Resting and electrical stimulation evoked[<sup>3</sup>H]5-HT and [<sup>3</sup>H]NA efflux in the hippocampus of P2rx7+/+ and P2rx7−/− mice, respectively.
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Tissue slices were loaded with [3H]5-HT or [3H]NA and then superfused with Krebs’ solution. After 60 min preperfusion, slices were stimulated electrically with the following parameters: 25 V, 1 msec, 2 Hz, 240 shocks. The release of radioactivity was expressed in Bq/g. For the calculation of the resting [3H]5-HT/[3H]NA efflux, the tritium content of the sample collected immediately before the first electrical stimulation period was taken into account. Electrical stimulation-induced [3H]5-HT/[3H]NA efflux (S1) was expressed by calculating the net release in response to electrical stimulation by the area-under-the-curve method. The number of experiments are in parentheses. *P<0.05, significantly different from P2rx7+/+ mice, calculated by the Student’s t-test.
