Effect of silencing of p53 on gene expression in MDA-MB 231 TNBC cell line. [sip53]

Mutant p53 can acquires oncogenic properties supporting tumor growth, metastases and chemoresistance, by reprogramming cancer cell transcriptome, proteome and metabolome. To investigate what is the gene expression network regulated by mutant p53, we silenced its expression in MDA-MB-231 Triple Negative Breast Cancer (TNBC) cell line. We then performed gene expression profiling analysis using data obtained from RNA-seq of MDA-MB 231. Overall design: Comparative gene expression profiling analysis of RNA-seq data for MDA-MB 231 cells transfected with Control or p53 siRNAs, each in triplicate.