Single-cell RNA Sequencing of Human Esophageal Epithelium in Homeostasis and Allergic Inflammation

To investigate gene expression signature of the human esophageal epithelium at the homeostatic and inflammatory conditions we performed scRNAseq on the human esophageal biopsies obtained from the healhy patients, patients with active eosinophilic esophagitis (EoE) and patients in the remission. Esophageal biopsies from obtained from the healhy patients, patients with active EoE and patients in the remission were collected, processed to generate single cell suspension and subjected to 10X scRNAseq