Machine learning analysis of ARVC informed by sodium channel protein-based interactome networks

The PKP2 mutation (PKP2+/-) rat as well as their wild type (WT) littermates (6-week-old) were obtained from Prof. Wang Xin (East China Normal University, Shanghai, China). All rats were fed in the Laboratory Animal Center of Putuo Hospital, Shanghai University of Traditional Chinese Medicine.Fresh 3 cardiac tissue was collected from each group with a surgical scissor to isolate single cells. The tissues were cut into small pieces of 0.5 mm3 in prechilled PBS with surgical scissors and digested with collagenase type II. The constructed library was sequenced using the Illumina Nova 6000 PE150 platform.