Transcriptome analysis of skeletal muscle from adipocyte-specific UCP1 knock-in (KI) pigs and wild-type (WT) pigs

In the present study, we conducted transcriptomics on skeletal muscle from 6-month-old UCP1 KI and wild-type (WT) pigs to explore the transcriptional programs induced by adipocyte-specific UCP1 overexpression in skeletal muscle We utilized transcriptomics to identify the regulatory pathways in skeletal muscles from adipocyte-specific UCP1 knock-in (KI) pigs. UCP1 KI changed the contents of glycerophospholipids and acyl carnitines of skeletal muscles. Several metabolic regulatory pathways were more enriched in the UCP1 KI skeletal muscle. Comparison of the transcriptomes of adipose and skeletal muscle suggested that nervous system or chemokine signaling might account for the crosstalk between these two tissues in UCP1 KI pigs. Our study reveals the transcriptional programs in the skeletal muscle of UCP1 KI pigs and suggests that a network regulates metabolic homeostasis between skeletal muscle and adipose tissue.