Transcriptome analysis of stomatal lineage ground cells (SLGCs)
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE129938
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Purpose: Identify SLGC cell fate by transcriptomes Methods: Cells expressing SLGC markers (BASLp:BRX-YFP, BASLp:myrBRX-YFP and myrBRXp:myrBRX-YFP) and stomatal commitment stage marker (MUTEp:MUTE-YFP) were collected by FACS. 9dpg plants were used for protoplasting and subjected to cell sorting. Around 20,000/sample were cellected and used for library preparation. Results: SLGCs are renriched in mitotic genes. In addition, top candidates, DEK and MYB16 proteins function in SLGC cell divisions. Conclusions: SLGCs are division competent cells and the coordination of cell cycle and transcriptional regulation though DEK is important for maintaining the transition (SLGC) state during stomatal development. SLGC mRNA profiles of 9dpg seedlings were generated by deep sequencing, in duplicates, using Illumina HiSeq.
创建时间:
2021-05-19



