Expression data from Malpighian tubules of wild type and dVHL +/- Drosophila 5-days old female

Mutations in VHL tumor suppressor gene are the cause of the familial VHL disease that displays multiple benign and malignant tumors such as clear cell renal cell carcinoma. The VHL gene is similar in Drosophila melanogaster (dVHL) and humans. We used microarrays to compare the gene expression profile between yellow white (used as control) and heterozygous dVHL +/- Malpighian tubes dissected from adult Drosophila female with the aim to identify distinct classes of up-regulated and downregulated genes in a sensitized (haploinsufficient) genetic background. Drosophila female of the appropriated genotype were transfered each day into new vials with supplemented yeast for 5 days. Malpighian tubules were then dissected for RNA extraction and hybridization on expression microarrays. Each sample was composed of Malpighian tubules dissected from 50 females of the same age.