Genome-wide excision repair map of UV-induced CPD damage in CSA1 and CSA2 knock-out Arabidopsis plants

We aimed to understand the roles of CSA1 and CSA2 proteins in transcription-coupled repair (TCR) in Arabidopsis and created csa1, csa2 and csa1csa2 double mutant plants. After irradiating 10-day old mutant seedlings with UVC, we generated genome-wide maps of cyclobutane pyrimidine dimer (CPD) damage repair in csa1, csa2 and csa1csa2 double mutant Arabidopsis plants using Excision Repair-sequencing (XR-seq) method. XR-seq libraries were sequenced using Illumina HiSeq 4000 platform, generating 50-nt single-end reads.