Detection of human CD45⁺/CD38⁺/CD34⁺ cells following serial transplantation.

Cohorts of NSG mice were irradiated with given a transplant of cord blood cells or G-CSF mobilized patient blood cells after a 12 day expansion with Tat-MYC/Tat-Bcl-2. The number of engrafted mice in each cohort is shown under 1st engraftment. Cohorts of NSG mice were irradiated with given a transplant of 106 bone marrow cells from primary xenochimaeric NSG mice. Mice were analyzed 8 weeks post-transplant for human CD45+/CD38+/CD34+ cells in their bone marrow compartment. The number of engrafted mice after serial transplant is shown under 2nd engraftment. Detection of human CD45+/CD38+/CD34+ cells following serial transplantation.