The major virulence factor of Plasmodium falciparum parasites, PfEMP1 is expressed by a multigene family, termed var genes. Here selection linked integration (SLI) was utilized to modify var genes in
The parasite Plasmodium falciparum is responsible for severe malaria, which remains a major cause of death, particularly in sub-Saharan Africa. The reference strain NF54 (or its subclone 3D7) is commo
The variant surface antigens expressed on Plasmodium falciparum–infected erythrocytes are potentially important targets of immunity to malaria and are encoded, at least in part, by a family of var gen
Additional file 6. List of DBLa tags from CM parasite lines at time of binding assay. DBLα tags from each parasite line one day prior to the HBMVEC cytoadherence experiments are reported. Total ring s
aThe ability of infected erythrocytes to float in 0.7% gelatin was used as a surrogate for knob positivity. bIndicates whether the knob-associated histidine rich protei