In vitro transcriptomic responses to thioacetamide-S-oxide exposure in Sprague-Dawley rat primary hepatocytes, renal tube epithelial, and cardiomyocytes

In this study we tested the ability to predict organ injury endpoints from in vitro transcriptomics responses at early time points (9 and 24 hours) after to thioacetamide-S-oxide treatment, the toxic metabolite of thioacetamide. Thioacetamide, an organosulfur compound, have been extensively used in animal studies as a hepatotoxin and carcinogen for its ability to cause acute liver damage. We treated three-cell types from Sprague-Dawley rats, primary hepatocytes (vehicle, low (0.025 mM), or high (0.125 mM) dose), renal tube epithelial cells (vehicle, low (0.125 mM), or high (0.500 mM) dose), and cardiomyocytes (vehicle, low (0.50 mM), or high (1.50 mM) dose) with thioacetamide-S-oxide. RNA-seq data were collected 9 and 24 hours after application of vehicle or thioacetamide-S-oxide.