Genomic inversion drives small colony variant formation and increased virulence in P. aeruginosa

Phenotypic change is a hallmark of bacterial adaptation during chronic infection. In the case of chronic Pseudomonas aeruginosa lung infection in patients with cystic fibrosis, well-characterised phenotypic variants include mucoid and small colony variants (SCVs). Here we show that P. aeruginosa SCVs can be reproducibly isolated from the murine lung following the establishment of chronic infection. Using a combination of single-molecule real-time (PacBio) and Illumina sequencing we identified the genetic switch for conversion to the SCV phenotype as a large genomic inversion through recombination between homologous regions of two rRNA operons. This phenotypic conversion was associated with large-scale transcriptional changes distributed through the genome. This global rewiring of the cellular transcriptomic output results changes to normally differentially regulated genes that modulate resistance to oxidative stress, central metabolism and virulence. These changes are of clinical relevance since the appearance of SCVs during chronic infection is associated with declining lung function