CITE-seq - MS Twin Study

We performed single-cell transcriptome, surface proteome and TCR analyses using 10X Chromium Single Cell Immune Profiling Solution (5’ approach) with feature barcoding. We successfully sequenced 8 selected twin pairs discordant for MS (16 individuals in total) + 2 additional healthy MS Twins. This allowed us to pair the high-throughput CyTOF data from 116 Twin samples with a high-resolution map of highly multiplexed surface protein expression and an unbiased in-depth analysis of the transcriptome (single-cell indexing of transcriptomes and epitopes: CITE-seq). Sequencing raw data and processed gene expression data have been deposited into the GEO repository under the accession numbers GSE193181.