TCR-Engine: Deep mutational scanning library selections
收藏NIAID Data Ecosystem2026-04-30 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP392328
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TnT cells reconstituted with DMS libraries and displaying restored TCR-CD3 surface expression were isolated by FACS, expanded and re-sorted based on binding to cognate peptide-MHC dextramer or activation following co-culture with cells displaying target antigen. Following FACS, deep sequencing was performed to identify TCR variants that were enriched across selection steps.
创建时间：
2022-08-19
