Eosinophils promote effector functions of lung ILC2s in allergic airway inflammation in mice

We investigated reciprocal interactions between cocultures of isolated populations of eosinophils and ILC2s and found these cells promote changes in gene expression as compared to being cultured alone. Pure populations of eosinophils and ILC2s were obtained from C57BL/6J mice and cultured alone or cocultured for 24 hours, then flow cytometry sorted to separate populations, followed by RNA-seq and gene expression profiling