RNA Sequencing on hippocampal tissue after ASO treatment overtime in MECP2 duplication mice. RNA Sequencing on hippocampal tissue after ASO treatment overtime in MECP2 duplication mice

To understand the response of MECP2-regulated genes to the ASO treatment, we performed RNA-sequencing at multiple timepoints on hippocampal tissue following ASO treatment. It shows that the abnormal transcriptomic profile of hDup mice is significantly rescued 2 weeks after the treatment. Long-term partial rescue of the gene expression was observed at 5 weeks and until 16 weeks after the treatment as the transcriptomic profile does not cluster with the control-ASO treated mice. Overall design: Hippocampal mRNA profiles of 1) hGFP mice with control ASO, 2) hDup mice with control ASO, and3) hDup mice with MECP2-ASO and harvest at different time points after ASO treatment