Gene deletion and reaction addition strategies identified by SimOptStrain for succinate production under glucose aerobic condition.

aEnzyme Commission number. 1.2.1.52 2-Oxoglutarate+CoA+NADP+< = >Succinyl-CoA+CO2+NADPH. 2.1.3.1 Malonyl-CoA+Pyruvate< = >Acetyl-CoA+Oxaloacetate. 1.2.1.51 Pyruvate+CoA+NADP+< = >Acetyl-CoA+CO2+NADPH. 1.4.1.20 L-Phenylalanine+H2O+NAD+< = >Phenylpyruvate+NH3+NADH. 1.4.1.9 L-Valine+H2O+NAD+< = >3-Methyl-2-oxobutanoate+NH3+NADH. bTheoretical maximum production (TMP) is reported as mol succinate produced/mol glucose consumed for each strain with non-native reaction additions, but without gene deletions. A maximum glucose uptake rate of 10 mmol gDW−1 h−1 and a maximum oxygen uptake rate of 18.5 mmol gDW−1 h−1 were used. cYield is reported as % of the TMP for each strain after reactions are added. dBest strategies without addition of non-native reactions.