Chromosome-level genome assembly and annotation of Pterygoplichthys pardalis

Suckermouth catfishes, with their evolved powerful features, have become notorious invasive species, causing significant damage to aquatic ecosystems. However, the lack of high-quality genomes severely restricts research on this group within the field. In this study, we de novo  assembled the chromosome-level genome assembly of Pterygoplichthys pardalis using multiple platforms of sequencing data, including Illumina short reads, Nanopore long reads, and Hi-C sequencing reads, resulting in a 1.51 Gb genome assembly. Multiple evaluations, including read mapping ratio (98.52%), transcript mapping ratio (99.61%), conserved BUSCO gene set (98.8%), and N50 score (49.47 Mb), indicated the high continuity and accuracy of the genome assembly we generated. Genome annotation found that 0.97 Gb of genome sequences are repetitive sequences, accounting for 64.47% of the genome assembly. Further, 23,859 protein-coding genes were successfully predicted, 92.92% of which could be annotated in functi..., , , , # Chromosome-level genome assembly and annotation of Pterygoplichthys pardalis

Dataset DOI: [10.5061/dryad.bk3j9kdgh](10.5061/dryad.bk3j9kdgh)

## Description of the data and file structure

Dataset DOI: [10.5061/dryad.bk3j9kdgh](10.5061/dryad.bk3j9kdgh)

The catfish samples used in this study were purchased from an ornamental fish wholesale market in Xi'an, China. The remaining samples of this specimen (Catfish_01) have been cryopreserved at -80°C in the Biodiversity Repository of the Institute of Basic and Translational Medicine at Xi'an Medical University. All animal specimens were collected legally in accordance with the policy of the Animal Care and Use Ethics of the institution. Genomic DNA was extracted from the muscle tissue of one suckermouth catfish (*P. pardalis*) using the Blood & Cell Culture DNA Mini Kit (Qiagen).

### Files and variables

#### File: Pterygoplichthys\\\_pardalis.cds

**Description:**  Gene coding sequence

#### File: Pterygoplichthys\\\_pardalis.pep

**De...