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CD249+ fibroblasts vs CD249 neg fibroblasts

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NIAID Data Ecosystem2026-03-13 收录
下载链接:
https://www.ncbi.nlm.nih.gov/sra/SRP279722
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资源简介:
Bulk sequencing of murine lung fibroblasts expressing a potential novel marker of lipofibroblasts (Enpep aka. Cd249). Overall design: Murine lungs were digested using conventional methods and a single cell suspension generated. Fibroblasts (CD45/CD326/CD31 negative) expressing Cd249 were flow sorted and collected. A equal number of CD249 negative fibroblasts from the same lung were collected at the same time. Total RNA was isolated using the Qiagen RNeasy Micro kit and stored at -80C until analyzed. All samples were submitted together submitted to the CSMC Genomics Core facilty for bulk RNA sequencing.
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2021-10-12
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