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High-accuracy meets high-throughput for microbiome profiling with near full-length 16S rRNA amplicon sequencing on the Nanopore platform

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NIAID Data Ecosystem2026-05-01 收录
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In this project, we present a UMI-based small sub-unit rRNA (e.g. 16S rRNA) amplicon sequencing method, termed ssUMI, that is both high-throughput and highly-accurate on the Nanopore platform using Q20+ chemistry, and showcased the high-throughput scalability of this method by sequencing 90 wastewater samples on a single PromethION flowcell for accurate microbial profiling.

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2023-05-19
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