Next Generation Sequencing Facilitates Quantitative Analysis of plasma Exosomal miRNAs of small cell lung cancer. Next Generation Sequencing Facilitates Quantitative Analysis of plasma Exosomal miRNAs of small cell lung cancer

Total RNA was isolated from plasma exosomes of two small cell lung cancer patients at the pre- and post-chemoresistant stages. MiRNA sequencing was performed to assess differential expression using next-generation sequencing. The data showed that most miRNAs levels were different in plasma exosomal. Importantly, miR-92b-3p were much higher in the post-chemoresistant stage than in the pre-chemoresistant stage Overall design: MiRNA sequencing was performed to assess differential expression of miRNAs in plasma exosomes of two small cell lung cancer patients at the pre- and post-chemoresistant stages using next-generation sequencing.