TOPBP1 Shapes XY Silencing Dynamics during Meiosis to Ensure Male Fertility

TOPBP1 and other DNA damage response proteins play an important role in meiotic sex chromosome inactivation. We investigate a mutant mouse with a mutation in the BRCT5 domain of TOPBP1 and use single-cell RNA-seq to investigate the effect of the mutation on XY silencing. Single-cell suspensions were generated from mouse testes and sorted by fluorescence-activated cell sorting to enrich for spermatocytes and analyzed using scRNA-seq (10X Genomics Chromium).