Small RNA sequencing of LA-N-2 and LAN-5 cells exposed to ciliary neurotrophic factor for 30/60 min and 2/4 days

To examine the impact of gp130-mediated, pro-cholinergic neurokine intervention on the small RNA systems in female and male human neuronal cells, we exposed cultured LA-N-2 cells (female) to 100 ng/ml ciliary neurotrophic factor (CNTF), and LAN-5 cells (male) to 10 ng/ml CNTF. RNA sequencing of time points at 30/60 minutes and 2/4 days of CNTF exposure shows a significant response and an “immediate early”/”long term” dichotomy in differentially expressed miRNAs. Overall design: Small RNA profiles were generated by Illumina sequencing (NextSeq) of 4 biological replicates each and analysed with miRExpress2.