Differential gene expression of RNA extracted from A549 cell line cultured with Amorphous Calcium Carboante (ACC) or without. Differential gene expression of RNA extracted from A549 cell line cultured with Amorphous Calcium Carboante (ACC) or without

Amorphous calcium carbonate (ACC) is a non-crystalline form of calcium carbonate, which is composed of aggregated nano-size primary particles. Here, we wanted to evaluate how ACC affects gene expression in a human lung cancer cell line (A549). Overall design: A549 cell line was cultured with DMEM/F12 medium (control) or with DMEM/F12 calcium delpeted medium to which 2mM elemntal calcium as ACC was added. Cells were cultred for 8 consecutive passages. After which, total RNA extraction was perfomred and samples went for RNAseq. Experiment was done in triplicates for eacg group (ACC or control) (N=6).