Scap Stabilizes PKM2 to Promote Glycolysis and Enhance Antifungal Immunity in Macrophages

Fungal infection induces substantial but poorly understood metabolic reprogramming in macrophages. To identify molecules that are potentially involved in the fungi infection of macrophages, RNA-Seq was conducted to compare the expression of mRNAs in Candida albicans infected BMDMs and control BMDMs from Scapf/f and Lyz2-cre-Scapf/f mice. Overall design: RNA-seq was performed on bone marrow–derived macrophages (BMDMs) stimulated with Candida albicans for 6 hours, with untreated cells used as controls from Scapf/f and Lyz2-cre-Scapf/f mice. Each group consisted of three samples, each containing 5 million cells. RNA extractions were sent to BGI (Shenzhen) for RNA-seq analysis.