Transcriptome analysis of naive B cells from CD19Cre and CD19Cre_STAT6vt mice. Transcriptome analysis of naive B cells from CD19Cre and CD19Cre_STAT6vt mice

To investigate how activated STAT6 modulates B cells development and function in vivo, we characterized mice that express a constitutively active version specifically in B cells. CD19Cre_STAT6vt mice show spontaneous phosphorylation and nuclear translocation of STAT6 in B cells. Based on the finding that STAT6vt accumulates in the nucleus of naïve splenic B cells in CD19Cre_STAT6vt mice, we next determined the changes of the global transcriptome by RNAseq analysis. Overall design: We isolated naïve B cells by cell sorting (CD19+ CD3neg PIneg, purity >95%) from three age matched CD19Cre_STAT6vt and three CD19Cre control mice, purified total RNA and performed transcriptome analysis using the Illumina platform.