Methylation comparison between ash tolerant and susceptible trees to ash dieback [methylation]

DNA was extracted from two ash tree samples, one tolerant and one susceptible to ash dieback. The DNA was sequenced using Nanopore technologies and the methylation was called against the new genome (BioProject PRJNA865134, SAMN30100368, genome JANJPF000000000 ) to identify differentially methylated regions between both samples. Manuscript title: Fraxinus excelsior updated long-read genome reveals the importance of MADS-box genes in tolerance mechanisms against ash dieback, G3:Genes|Genomes|Genetics DNA was extracted using the method of Workman et al., (Workman et al., 2018) from a pair of Danish trees previously described in Harper et al. (Harper et al., 2016): association panel tree Ash66, and prediction panel tree DNASH35, which had ash dieback disease damage scores of 0 and 75% respectively. The libraries were prepared using the SQK-LSK109 ligation sequencing kit and run with PromethION R9.4.1 (FLO-PRO002) flow cells, base calling was generated automatically. Nanopolish was used to call the methylation profile of each sample.