Sequences of a set of polyadenylated transcripts belonging to the protist Leishmania infantum (JPCM5 strain) that were determined by PacBio sequencing.. Characterization and annotation of Leishmania infantum (JPCM5 strain) transcript isoforms.

Total RNA from Leishmania infantum (JPCM5 strain) promastigotes was retrotranscribed using poli-T oligonucleotide into cDNA. After a step of PCR amplification, the resulting DNA was sequenced using the Single Molecule, Real-Time (SMRT) Sequencing methodology (PacBio). High quality reads were used to generate circular consensus sequences (CCS), which were classified as complete or truncated transcripts, depending on the presence at 5’-end of the mini-exon (spliced-leader) sequence or not, respectively. Here, we are reporting the sequences of all the transcript isoforms generated in this study.