Throat swab targeted sequencing data from 214 respiratory tract infection patients

A study was conducted on patients with suspected respiratory tract infections who presented at Longgang District People's Hospital of Shenzhen between February 2023 and June 2023. Oropharyngeal swab samples were collected from eligible patients by healthcare professionals. Following collection, oropharyngeal swabs were transported to the clinical laboratory within 2 hours. Co-extraction of DNA and RNA from the samples was performed using an automated nucleic acid extraction system. Sequencing libraries were subsequently constructed using a commercially available respiratory pathogen panel targeted sequencing kit, according to the manufacturer's instructions. The prepared libraries were sequenced on either an Illumina Miniseq or Nextseq 500 platform at the Shenzhen center for disease control and prevention.