Columba livia isolate:Cliv_WK_1.0 Genome sequencing and assembly

We used a combined strategy to generate a domestic pigeon (Columba livia) reference genome. This entailed using 86.75 Gb of long-read data from single-molecule real-time (SMRT) sequencing (Pacbio) to assemble contig sequences, 84 Gb of high-fidelity short-read data (Illumina) for genome-wide base-level corrections, and 213 Gb of data from high-throughput chromosome conformation capture (Hi-C) sequencing to build chromosome-­level super­-scaffolds. The assembled genome was ~1.18 Gb, with contig and scaffold N50 lengths of 7.79 Mb and 35.84 Mb, respectively.