In vivo analysis of camptothecin effect in primary DRG neuron after sciatic nerve injury

Analysis of gene expression in injured primary DRG with or without camptothecin (CPT) treatment after sciatic nerve crushing may help us identify critical molecular pathways related to axon regeneration. We performed RNA-sequencing of (i) Naive primary DRG tissues without injury, (ii) Primary DRG tissues with vehicle treatment different time-points (18, 24, 36 hours) after sciatic nerve injury, and (iii) Primary DRG tissues with camptothecin treatment different time-points (18, 24, 36 hours) after sciatic nerve injury. Overall design: 12 weeks-old wild-type C57/BL6 mice underwent sciatic nerve crush (SNC). Thirty minutes after crush, 2 mg/kg of camptothecin was administered to injured mice by intraperitoneal injection. DRG tissues were harvested at 18, 24, and 36 hours after nerve injury either from camptothecin injected group or vehicle-injected group (n=4 at each time point).